Stem cells and Neurodegenerative diseases

Summary

The main focus of our laboratory is to study basic biological aspects underlying neurodegenerative diseases with complex genetic etiologies, such as Parkinson’s disease, as well as inherited metabolic brain diseases. We apply human reprogramming technologies , mainly induced pluripotent stem cells (iPSCs) together with gene editing technology, to develop the next generation of human disease models. By combining basic cellular neuroscience approaches with sequencing-based data biology, the goal of our laboratory is to identify the key molecular drivers of Parkinson's disease to enable the development of diagnostic tools and novel therapeutics approaches for this and other neurodegenerative diseases.

135 Publications

Stem-cells-and-Neurodegenerative-diseases

Strategic Lines

Analysis of the pathogenic mechanisms that lead to neurodegeneration of DAn derived from patient-specific iPSC (rescue of PD-related neurodegeneration)
Identification of early functional alterations in the differentiated DA of the iPSC of the patient with specific PD (prevention of neurodegeneration related to PD)
Investigate the role of the genetic makeup of PD patients who carry pathogenic mutations in the development of PD-related neurodegeneration (cause of PD vs. predisposition)
Study the cross-talk between patient-specific iPSC-derived DAn and glial cells

Selected Publications

Enhancing glycolysis attenuates Parkinson's disease progression in models and clinical databases

Cai R., Zhang Y., Simmering J.E., Schultz J.L., Li Y., Fernandez-Carasa I., Consiglio A., Raya A., Polgreen P.M., Narayanan N.S., Yuan Y., Chen Z., Su W., Han Y., Zhao C., Gao L., Ji X., Welsh M.J., Liu L.

JOURNAL OF CLINICAL INVESTIGATION (ISSN: 0021-9738) 2019

Global Proteomic and Methylome Analysis in Human Induced Pluripotent Stem Cells Reveals Overexpression of a Human TLR3 Affecting Proper Innate Immune Response Signaling.

Requena J, Alvarez-Palomo AB, Codina-Pascual M, Delgado-Morales R, Moran S, Esteller M, Sal M, Juan M, Boronat Barado A, Consiglio A, Bogle OA, Wolvetang E, Ovchinnikov D, Alvarez I, Jaraquemada D, Mezquita-Pla J, Oliva R, Edel MJ

STEM CELLS (ISSN: 1549-4918) 2019

Patient-Specific iPSC-Derived Astrocytes Contribute to Non-Cell-Autonomous Neurodegeneration in Parkinson's Disease.

di Domenico A, Carola G, Calatayud C, Pons-Espinal M, Muñoz JP, Richaud-Patin Y, Fernandez-Carasa I, Gut M, Faella A, Parameswaran J, Soriano J, Ferrer I, Tolosa E, Zorzano A, Cuervo AM, Raya A, Consiglio A

STEM CELL REPORTS (ISSN: 2213-6711) 2019

Whole-genome DNA hyper-methylation in iPSC-derived dopaminergic neurons from Parkinson's disease patients.

Fernández-Santiago R, Merkel A, Castellano G, Heath S, Raya Á, Tolosa E, Martí MJ, Consiglio A, Ezquerra M

CLINICAL EPIGENETICS (ISSN: 1868-7075) 2019

CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons

Calatayud C., Carola G., Fernández-Carasa I., Valtorta M., Jiménez-Delgado S., Díaz M., Soriano-Fradera J., Cappelletti G., García-Sancho J., Raya Á., Consiglio A.

SCIENTIFIC REPORTS (ISSN: 2045-2322) 2019

Selected Projects