Investigadors del grup de recerca en Sarcomes de l\u2019Institut d\u2019Investigaci\u00f3 Biom\u00e8dica de Bellvitge (IDIBELL), liderats pel Dr. \u00d2scar Mart\u00ednez-Tirado, han descrit per primera vegada el perfil de metilaci\u00f3 del sarcoma d\u2019Ewing (SE), un c\u00e0ncer de teixit ossi i tou que afecta principalment a nens i adolescents. El seu analisi els ha perm\u00e8s identificar el potencial del gen PTRF com a marcador pron\u00f2stic de la malaltia i com a possible diana ter\u00e0peutica futura en conjunt amb les noves eines d\u2019edici\u00f3 gen\u00f2mica. <\/p>\n
\u201c\u00c9s la primera vegada que es descriu el perfil de metilaci\u00f3 del sarcoma d\u2019Ewing\u201d, comenta el Dr. \u00d2scar Mart\u00ednez-Tirado, l\u00edder del treball. La metilaci\u00f3 de l’ADN \u00e9s un tipus de modificaci\u00f3 epigen\u00e8tica capa\u00e7 de controlar l’expressi\u00f3 g\u00e8nica, \u00e9s a dir, pot activar o desactivar gens que donen lloc o bloquegen caracter\u00edstiques i processos en els individus, entre ells el desenvolupament de tumors. Mitjan\u00e7ant eines bioinform\u00e0tiques, l\u2019equip de recerca va buscar elements comuns que els permetessin relacionar el conjunt de gens hipometilats o hipermetilats de forma diferencial en les mostres tumorals. <\/p>\n
Els investigadors van identificar un grup de 12 gens relacionats amb l\u2019estructura de la membrana cel\u00b7lular; entre ells, el precursor de la prote\u00efna PTRF va cridar especialment l\u2019atenci\u00f3 dels investigadors per la seva relaci\u00f3 amb les caveoles, unes petites invaginacions presents en la membrana d\u2019algunes c\u00e8l\u00b7lules; el grup de l\u2019IDIBELL t\u00e9 \u00e0mplia experi\u00e8ncia en l\u2019estudi d\u2019aquestes estructures i la seva relaci\u00f3 amb el SE. <\/p>\n
L\u2019equip va relacionar la pres\u00e8ncia de PTRF en mostres de 67 pacients. \u201cInicialment, vam veure que aquells pacients que expressen PTRF tenen una millor superviv\u00e8ncia; per tant, podr\u00edem considerar aquesta prote\u00efna com a marcador potencial del pron\u00f2stic de la malaltia\u201d, conclou Mart\u00ednez-Tirado. <\/p>\n
Les c\u00e8l\u00b7lules normals amb caveoles expressen tant la prote\u00efna PTRF com caveolina-1 (CAV1), una altra prote\u00efna relacionada amb la formaci\u00f3 de caveoles. En canvi, les c\u00e8l\u00b7lules tumorals en SE ni tenen caveoles, ni expressen PTRF. \u201cAix\u00f2 ens va fer pensar en la possibilitat de reintroduir de forma ex\u00f2gena el gen precursor de PTRF en les l\u00ednies cel\u00b7lulars d\u2019estudi\u201d, explica l\u2019investigador. \u201cEn aquelles que tamb\u00e9 expressaven CAV1, la reintroducci\u00f3 de PTRF va desencadenar la formaci\u00f3 de caveoles. Per a les c\u00e8l\u00b7lules tumorals, aquesta modificaci\u00f3 de l\u2019estructura resulta tan estressant que les destrueix\u201d. A mes, els investigadors han demostrat que la formaci\u00f3 de la caveola en aquestes c\u00e8l\u00b7lules activa una coneguda via de senyalitzaci\u00f3 de mort cel\u00b7lular promoguda per la prote\u00efna p53. <\/p>\n
Els f\u00e0rmacs epigen\u00e8tics actuals s\u00f3n for\u00e7a inespec\u00edfics, per\u00f2 les noves eines d\u2019edici\u00f3 gen\u00f2mica CRISPR tenen el potencial d\u2019utilitzar-se per desmetilar gens espec\u00edfics d\u2019una c\u00e8l\u00b7lula tumoral en un futur. \u201cSi som capa\u00e7os d\u2019actuar de forma espec\u00edfica sobre el gen promotor de PTRF de manera que la prote\u00efna s\u2019expressi a nivells normals, induir\u00edem la mort cel\u00b7lular i per tant estar\u00edem davant d\u2019una nova i prometedora opci\u00f3 terap\u00e8utica personalitzada per al SE\u201d. <\/p>\n
Aquest ha estat un treball altament col\u00b7laboratiu, tant a nivell investigador, on s\u2019ha comptat amb el suport de dos grups del programa d\u2019epigen\u00e8tica i biologia del c\u00e0ncer de l\u2019IDIBELL, l\u2019Institut Germans Trias, l\u2019Institut de Biomedicina de Sevilla, l\u2019Hospital Universitari La Paz, l\u2019Hospital Infantil Universitari Ni\u00f1o Jes\u00fas, l\u2019Hospital Sant Joan de D\u00e9u, l\u2019Hospital Universitari Vall d\u2019Hebron i l\u2019Hospital Universitari Virgen del Roc\u00edo, com a nivell econ\u00f2mic, ja que el projecte rep finan\u00e7ament per part de l\u2019Institut de Salut Carlos III, l\u2019AECC i la Fundaci\u00f3n Alba P\u00e9rez.<\/p>\n","protected":false},"excerpt":{"rendered":"
Investigadors del grup de recerca en Sarcomes de l\u2019Institut d\u2019Investigaci\u00f3 Biom\u00e8dica de Bellvitge (IDIBELL), liderats pel Dr. \u00d2scar Mart\u00ednez-Tirado, han descrit per primera vegada el perfil de metilaci\u00f3 del sarcoma d\u2019Ewing (SE), un c\u00e0ncer de teixit ossi i tou que afecta principalment a nens i adolescents. El seu analisi els ha perm\u00e8s identificar el potencial […]<\/p>\n","protected":false},"author":6,"featured_media":10715,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-7883","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sin-categorizar"],"publishpress_future_action":{"enabled":false,"date":"2024-12-26 01:05:02","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7883","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/comments?post=7883"}],"version-history":[{"count":0,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7883\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media\/10715"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media?parent=7883"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/categories?post=7883"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/tags?post=7883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}