La t\u00e8cnica de CRISPR est\u00e0 revolucionant la investigaci\u00f3 biom\u00e8dica, ja que permet editar els genomes de forma molt precisa. Tot i que el CRISPR permet generar o corregir mutacions d\u2019un o pocs nucle\u00f2tids amb certa facilitat, aquesta t\u00e8cnica encara t\u00e9 limitacions a l\u2019hora d\u2019introduir en el genoma fragments d\u2019ADN m\u00e9s llargs. Aix\u00ed, per exemple, la inserci\u00f3 en el genoma d\u2019un gen que produeixi una prote\u00efna fluorescent com la GFP, d\u2019ampli \u00fas en investigaci\u00f3, \u00e9s poc eficient i utilitza plasmidis que requereixen clonatges complexos.<\/p>\n
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El grup del Dr. Cer\u00f3n ha utilitzat l\u2019animal model Caenorhabditis elegans per optimitzar l\u2019\u00fas d\u2019aquesta t\u00e8cnica i ha desenvolupat el m\u00e8tode Nested CRISPR. Aquest m\u00e8tode, lliure de clonatges, insereix fragments d\u2019ADN en dos passos. En un primer pas introdueix en el genoma una petita part (menys de 200 nucle\u00f2tids) del fragment llarg, i aquesta petita seq\u00fc\u00e8ncia serveix de \u201cniu\u201d o \u201cpista d\u2019aterratge\u201d per a qu\u00e8 el fragment m\u00e9s llarg (d\u2019una quilobase aproximadament) s\u2019introdueixi en el genoma en un segon pas de forma eficient.<\/p>\n
\u00a0<\/p>\n
El treball, publicat recentment a la revista Genetics, de la Societat Americana de Gen\u00e8tica, est\u00e0 despertant un inter\u00e8s extraordinari, assolint la m\u00e0xima puntuaci\u00f3 en els \u00edndex de repercussi\u00f3 de la revista. Els animals model com C. elegans, amb un cicle de vida curt, permeten als cient\u00edfics investigar sobre les possibilitats i les limitacions del CRISPR. Aquest estudi forma part de la tesi doctoral de Jeremy Vicencio, que t\u00e9 la prestigiosa beca INPhINIT de La Caixa. Tant ell com les investigadores predoctorals Carmen Mart\u00ednez i X\u00e8nia Serrat, han realitzat centenars de microinjeccions en la l\u00ednia germinal de C. elegans i milers de genotipats per demostrar d\u2019una manera s\u00f2lida i convincent l\u2019efici\u00e8ncia de la nova t\u00e8cnica Nested CRISPR.<\/p>\n
\u00a0<\/p>\n
Com a mol\u00e8cules reparadores d\u2019ADN, el Nested CRISPR utilitza oligonucle\u00f2tids comercials per al primer pas i productes de PCR universals per al segon. Aix\u00f2 facilita experiments a gran escala que permetrien etiquetar centenars de gens amb prote\u00efnes fluorescents d\u2019inter\u00e8s. La t\u00e8cnica de Nested CRISPR tamb\u00e9 \u00e9s modular, ja que permet afegir altres p\u00e8ptids o prote\u00efnes d\u2019inter\u00e8s a les prote\u00efnes fluorescents. Aix\u00ed, per exemple, el grup est\u00e0 treballant en el marcatge d\u2019aquestes prote\u00efnes fluorescents amb p\u00e8ptids que permetin la seva degradaci\u00f3 d\u2019una forma controlada per l\u2019investigador.\u00a0<\/p>\n
\u00a0<\/p>\n
Finalment, el grup de models de malaltia en C. elegans, dirigit pel Dr. Cer\u00f3n, pret\u00e9n utilitzar el Nested CRISPR per substituir gens de C. elegans amb gens humans. D\u2019aquesta manera, aquest petit nematode podria servir de model multicel\u00b7lular per estudiar l\u2019efecte de mutacions humanes relacionades amb malalties. Aquest sistema tindria for\u00e7a inter\u00e8s en el camp de la medicina personalitzada ja que d\u2019una forma r\u00e0pida i eficient es podria tenir un pron\u00f2stic sobre la patogenicitat d\u2019una mutaci\u00f3 o d\u2019una variaci\u00f3 del genoma (conegudes com a polimorfismes).
\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"
La t\u00e8cnica de CRISPR est\u00e0 revolucionant la investigaci\u00f3 biom\u00e8dica, ja que permet editar els genomes de forma molt precisa. Tot i que el CRISPR permet generar o corregir mutacions d\u2019un o pocs nucle\u00f2tids amb certa facilitat, aquesta t\u00e8cnica encara t\u00e9 limitacions a l\u2019hora d\u2019introduir en el genoma fragments d\u2019ADN m\u00e9s llargs. Aix\u00ed, per exemple, la […]<\/p>\n","protected":false},"author":6,"featured_media":7516,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[334,355],"tags":[],"class_list":["post-7515","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicina-translacional","category-models-de-malaltia-en-c-elegans"],"publishpress_future_action":{"enabled":false,"date":"2024-12-29 10:39:49","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7515","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/comments?post=7515"}],"version-history":[{"count":0,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7515\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media\/7516"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media?parent=7515"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/categories?post=7515"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/tags?post=7515"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}