M\u00e9s sensible no \u00e9s sempre millor. En el seu darrer treball a Annals of Oncology, un equip nacional de recerca liderat per investigadors de l\u2019Institut d\u2019Investigaci\u00f3 Biom\u00e8dica de Bellvitge (IDIBELL) – Institut Catal\u00e0 d\u2019Oncologia (ICO) ha demostrat que la utilitzaci\u00f3 de noves t\u00e8cniques d\u2019an\u00e0lisi mutacional que permeten la quantificaci\u00f3 d\u2019al\u00b7lels mutats no millora el rendiment predictiu de les t\u00e8cniques emprades actualment. L\u2019estudi presenta els resultats de l\u2019assaig cl\u00ednic de fase II ULTRA, liderat pel Dr. Ramon Salazar, coordinador del programa Oncobell, i reafirma la validesa dels estudis mutacionals actuals.\u00a0<\/p>\n
\u00a0<\/p>\n
Les ter\u00e0pies actuals en estadis avan\u00e7ats de c\u00e0ncer de c\u00f2lon i recte inclouen la utilitzaci\u00f3 d\u2019anticossos monoclonals contra el receptor del factor de creixement epid\u00e8rmic EGFR (panitumumab, cetuximab). Aquests f\u00e0rmacs s\u00f3n capa\u00e7os de bloquejar l\u2019activaci\u00f3 de la via de senyalitzaci\u00f3 molecular EGFR, que es vincula a la proliferaci\u00f3 descontrolada de les c\u00e8l\u00b7lules canceroses. No obstant, quan els gens implicats en l\u2019activaci\u00f3 de la via, pertanyents a la fam\u00edlia RAS, presenten mutacions, la via no respon a l\u2019actuaci\u00f3 d\u2019aquests inhibidors; es tracta d\u2019un cas de farmacoresist\u00e8ncia.\u00a0<\/p>\n
\u00a0<\/p>\n
\u201cAquest fet, no obstant, ens aporta informaci\u00f3 cl\u00ednicament \u00fatil: \u00a0la identificaci\u00f3 d\u2019aquestes mutacions de RAS ens permet identificar els pacients que no respondran a aquests f\u00e0rmacs\u201d, explica la doctora Cristina Santos, primera autora de l\u2019estudi. \u201cEn el nostre estudi, vol\u00edem anar m\u00e9s enll\u00e0 i avaluar si la quantitat d\u2019al\u00b7lels mutats en el tumor correlaciona amb la resist\u00e8ncia prim\u00e0ria als f\u00e0rmacs\u201d, afegeix.<\/p>\n
\u00a0<\/p>\n
L\u2019assaig, en qu\u00e8 van participar 72 pacients de c\u00e0ncer colorectal metast\u00e0tic, va analitzar 38 punts susceptibles de patir mutacions en els gens KRAS, NRAS, BRAF i PIK3CA. L\u2019equip de recerca IDIBELL-ICO va identificar els perfils de mutaci\u00f3 tant per PCR quantitativa (qPCR), el m\u00e8tode habitualment emprat per fer aquestes determinacions, com per PCR digital (dPCR) nanoflu\u00eddica, un m\u00e8tode d\u2019alta sensibilitat capa\u00e7 de detectar i quantificar mutacions presents en un percentatge m\u00e9s baix de l\u20191% de la poblaci\u00f3 cel\u00b7lular. Ambd\u00f3s resultats es van correlacionar amb els resultats cl\u00ednics de cada pacient per tal d\u2019estimar el rendiment predictiu de cada t\u00e8cnica.\u00a0<\/p>\n
\u00a0<\/p>\n
\u201cEl que hem vist \u00e9s que, malgrat la dPCR d’alta sensibilitat va identificar un major nombre de \u00a0mutacions dels gens KRAS, NRAS, BRAF i PIK3CA presents en percentatges inferiors en el tumor, el llindar de sensibilitat \u00f2ptim per a la predicci\u00f3 de resist\u00e8ncia \u00e9s del 5%, per la qual cosa la qPCR tradicional \u00e9s suficientment sensible per a aquestes determinacions en el teixit tumoral\u201d, explica la Dra. Santos.\u00a0<\/p>\n
\u00a0<\/p>\n
L\u2019estudi, que ha rebut el suport del Grup de tractament de tumors digestius (TTD) a trav\u00e9s d\u2019un ajut del Ministeri de Salut, Afers Socials i Igualtat, ha estat coordinat pels doctors i investigadors IDIBELL-ICO Ramon Salazar i Gabriel Capell\u00e1. Hi ha participat 12 hospitals a nivell nacional; l\u2019Institut Catal\u00e0 d\u2019Oncologia n\u2019ha estat un dels principals centres reclutadors.\u00a0
\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"
Investigadors de l\u2019IDIBELL i de l\u2019ICO han demostrat que les t\u00e8cniques actuals d\u2019an\u00e0lisi mutacional s\u00f3n suficientment sensibles per a predir l\u2019aparici\u00f3 de resist\u00e8ncia al tractament amb inhibidors EGFR en c\u00e0ncer colorectal metast\u00e0tic<\/p>\n","protected":false},"author":6,"featured_media":7498,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[332,375,417],"tags":[],"class_list":["post-7497","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cancer","category-colorectal","category-cancer-hereditari-noticies"],"publishpress_future_action":{"enabled":false,"date":"2024-11-11 23:30:54","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7497"}],"collection":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/comments?post=7497"}],"version-history":[{"count":0,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7497\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media\/7498"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media?parent=7497"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/categories?post=7497"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/tags?post=7497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}