Un grup de cient\u00edfics encap\u00e7alat per la Dra. Antonella Consiglio de l\u2019IDIBELL i la Universitat de Barcelona (UB), i el Dr. Angel Raya del Centre de Medicina Regenerativa de Barcelona (CMR[B]\/IDIBELL) revela avui a la revista Scientific Reports un nou m\u00e8tode eficient per generar neurones dopamin\u00e8rgiques marcades amb fluoresc\u00e8ncia (DAn), un tipus espec\u00edfic de c\u00e8l\u00b7lula que mor\u00a0en la malaltia de Parkinson (PD), i que facilitar\u00e0 la futura recerca en processos associats amb la biologia de les DAn i de la malaltia.<\/p>\n
La malaltia de Parkinson (PD) \u00e9s un trastorn neurodegeneratiu incurable en la gent gran, que es caracteritza per la progressiva p\u00e8rdua de neurones dopamin\u00e8rgiques (DAn) a la subst\u00e0ncia negra del cervell. La seva incid\u00e8ncia als pa\u00efsos occidentals est\u00e0 incrementant degut a l\u2019augment de l\u2019esperan\u00e7a de vida de la poblaci\u00f3, suposant una major despesa pels sistemes sanitaris nacionals. Despr\u00e9s de d\u00e8cades d\u2019intensa investigaci\u00f3, els mecanismes pat\u00f2gens de la malaltia de Parkinson encara romanen desconeguts.<\/p>\n
El laboratori de la Dra. Consiglio utilitza c\u00e8l\u00b7lules mare pluripotents indu\u00efdes (iPSC) a partir de c\u00e8l\u00b7lules de la pell dels pacients, i les diferencia a tipus cel\u00b7lulars rellevants per la malaltia, com serien les neurones dopamin\u00e8rgiques en el cas de la PD. Aix\u00f2 ofereix una oportunitat sense precedents per modelar in vitro les causes que inicien el proc\u00e9s patol\u00f2gic de la PD, i per provar medicaments d\u2019una manera sistematitzada i amb un alt rendiment.<\/p>\n
Tot i aix\u00ed, les proves amb medicaments es veuen limitades per l\u2019heterogene\u00eftat dels cultius dopamin\u00e8rgics derivats a partir de c\u00e8l\u00b7lules mare pluripotents humanes, a m\u00e9s de la incapacitat per visualitzar aquestes c\u00e8l\u00b7lules dopamin\u00e8rgiques vives. \u201cPer esquivar aquesta barrera t\u00e8cnica, hem generat una l\u00ednia cel\u00b7lular fluorescent de iPSC amb el gen reporter de la Tirosina Hidroxilasa (TH), utilitzant el modificador de gens CRISPR\/Cas9 per inserir la prote\u00efna fluorescent a la part del genoma del gen dopamin\u00e8rgic espec\u00edfic (TH)\u201d, diu el Dr. Carles Calatayud, post-doc al laboratori de la Dra. Consiglio.<\/p>\n
\u201cAquest reporter TH de iPSC descrit al nostre treball, permet visualitzar neurones dopamin\u00e8rgiques vives per fluoresc\u00e8ncia en poblacions neurals barrejades, i purificar-les a trav\u00e9s de citometria de flux. Aquest fet ja s\u2019havia intentat pr\u00e8viament en altres estudis\u201d comenta A. Consiglio, \u201cper\u00f2 l\u2019expressi\u00f3 del gen reporter (TH) \u00e9s massa baixa per detectar-la en directe\u201d. \u201cAl nostre estudi hem utilitzat una prote\u00efna fluorescent m\u00e9s brillant (mOrange), que ha compensat la baixa expressi\u00f3 de TH\u201d, afegeix C. Calatayud.<\/p>\n
En col\u00b7laboraci\u00f3 amb el professor Jordi Soriano, del Departament de F\u00edsica de la Universitat de Barcelona (UB), aquesta l\u00ednia gen\u00e8ticament dissenyada va ser tamb\u00e9 usada per revelar trets funcionals \u00fanics de les neurones dopamin\u00e8rgiques, demostrant l\u2019important potencial d\u2019aquesta eina per futurs estudis orientats a l\u2019enteniment de la vulnerabilitat dopamin\u00e8rgica, a la patogenicitat de la PD i a possibilitar proves d\u2019alt rendiment.<\/p>\n
Altres investigadors de l\u2019estudi han estat Giulia Carola, Irene Fernandez-Carasa de l\u2019IDIBELL\/UB; Giulia Marco Valtorta, Graziella Cappelletti del departament de Bioci\u00e8ncia de la Universitat de Mil\u00e0 (Mil\u00e0); Senda Jim\u00e9nez, M\u00f2nica Diez del CMR[B]\/IDIBELL (Barcelona) i Javier Garc\u00eda-Sancho de l\u2019Institut de Biologia i Gen\u00e8tica Molecular (IBGM) de la Universitat de Valladolid (Valladolid).<\/p>\n","protected":false},"excerpt":{"rendered":"
Un grup de cient\u00edfics encap\u00e7alat per la Dra. Antonella Consiglio de l\u2019IDIBELL i la Universitat de Barcelona (UB), i el Dr. Angel Raya del Centre de Medicina Regenerativa de Barcelona (CMR[B]\/IDIBELL) revela avui a la revista Scientific Reports un nou m\u00e8tode eficient per generar neurones dopamin\u00e8rgiques marcades amb fluoresc\u00e8ncia (DAn), un tipus espec\u00edfic de c\u00e8l\u00b7lula […]<\/p>\n","protected":false},"author":6,"featured_media":10492,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[353,349,340,483],"tags":[],"class_list":["post-7481","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cellules-mare-i-malalties-neurodegeneratives","category-medicina-regenerativa","category-neurociencies","category-potencia-de-cellules-mare"],"publishpress_future_action":{"enabled":false,"date":"2024-12-02 03:08:53","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7481","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/comments?post=7481"}],"version-history":[{"count":0,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7481\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media\/10492"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media?parent=7481"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/categories?post=7481"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/tags?post=7481"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}