{"id":7319,"date":"2020-03-31T00:00:00","date_gmt":"2020-03-30T22:00:00","guid":{"rendered":"https:\/\/idibell.cat\/blog\/2020\/03\/31\/un-estudi-pilot-de-sequenciacio-de-la-microbiota-intestinal-assenta-les-bases-per-a-la-recerca-de-marcadors-de-deteccio-precoc-de-cancer-de-colon\/"},"modified":"2020-05-14T17:29:42","modified_gmt":"2020-05-14T15:29:42","slug":"un-estudi-pilot-de-sequenciacio-de-la-microbiota-intestinal-assenta-les-bases-per-a-la-recerca-de-marcadors-de-deteccio-precoc-de-cancer-de-colon","status":"publish","type":"post","link":"https:\/\/idibell.cat\/2020\/03\/un-estudi-pilot-de-sequenciacio-de-la-microbiota-intestinal-assenta-les-bases-per-a-la-recerca-de-marcadors-de-deteccio-precoc-de-cancer-de-colon\/","title":{"rendered":"Un estudi pilot de seq\u00fcenciaci\u00f3 de la microbiota intestinal assenta les bases per a la recerca de marcadors de detecci\u00f3 preco\u00e7 de c\u00e0ncer de c\u00f2lon"},"content":{"rendered":"

La microbiota intestinal, \u00e9s a dir, els microorganismes que viuen en els nostres intestins, ens poden donar pistes de l\u2019estat de salut d\u2019una persona, ja que la seva composici\u00f3 pot dependre de factors com la dieta, l\u2019estil de vida o de les patologies que patim. Es m\u00e9s, saber quins bacteris concrets estan als nostres intestins ens podria ajudar a predir malalties com el c\u00e0ncer de c\u00f2lon. Els nous aven\u00e7os en els m\u00e8todes de seq\u00fcenciaci\u00f3 del genoma i les eines bioinform\u00e0tiques que ens permeten analitzar-ne les dades, ens han ajudat a identificar milers de nous microorganismes presents als nostres intestins a trav\u00e9s de l\u2019an\u00e0lisi del seu genoma.<\/p>\n

Un equip format per investigadors de l\u2019Institut d\u2019Investigaci\u00f3 Biom\u00e8dica de Bellvitge (IDIBELL) i de l\u2019Institut Catal\u00e0 d\u2019Oncologia (ICO), han realitzat una prova pilot en l\u2019an\u00e0lisi del genoma de la microbiota intestinal. En aquest estudi han analitzat, per dos m\u00e8todes de seq\u00fcenciaci\u00f3 diferents, bi\u00f2psies de c\u00f2lon i mostres fecals de nou pacients. L\u2019objectiu ha estat posar a punt\u00a0 les t\u00e8cniques de seq\u00fcenciaci\u00f3 i les eines d\u2019an\u00e0lisi bioinform\u00e0tic.<\/p>\n

Es tracta del primer treball d\u2019un projecte que pret\u00e9n ser molt m\u00e9s extens. Les dades obtingudes en aquesta prova pilot serviran de base per al disseny del m\u00e8tode d\u2019an\u00e0lisi del projecte Colonbiome<\/a>. Aquest gran projecte t\u00e9 per objectiu buscar marcadors de la microbiota que puguin servir per a la detecci\u00f3 preco\u00e7 del c\u00e0ncer de c\u00f2lon. Per fer-ho, es recolliran bi\u00f2psies de c\u00f2lon i mostres de femta de pacients sans i pacients amb diferents fases de c\u00e0ncer colorectal, despr\u00e9s se\u2019n seq\u00fcenciar\u00e0 el genoma de la microbiota per identificar les difer\u00e8ncies entre els grups.<\/p>\n

 <\/p>\n

Dades a l\u2019abast de tothom<\/h4>\n

 <\/p>\n

Totes les dades obtingudes en aquest estudi pilot han estat introdu\u00efdes en la European Nucleotide Archive, una base de dades p\u00fablica i col\u00b7laborativa, on es comparteixen seq\u00fc\u00e8ncies gen\u00f2miques de tota mena per a l\u2019aprofitament de tota la comunitat cient\u00edfica. A m\u00e9s, tots els resultats d\u2019aquesta primera prova pilot han estat publicats a la revista Scientific Data, revista tamb\u00e9 p\u00fablica, on s\u2019hi han detallat tant les seq\u00fc\u00e8ncies com les f\u00f3rmules d\u2019an\u00e0lisi bioinform\u00e0tic utilitzades.<\/p>\n

Aquest estudi no nom\u00e9s pret\u00e9n ser \u00fatil per als futurs treballs del grup, sin\u00f3 que gr\u00e0cies al seu car\u00e0cter obert i p\u00fablic pret\u00e9n ser una ajuda a tots aquells grups de recerca que estiguin realitzant an\u00e0lisis similars o provant noves eines bioinform\u00e0tiques, als quals se\u2019ls posa a l\u2019abast tots els resultats obtinguts. A m\u00e9s, els investigadors asseguren que tamb\u00e9 faran p\u00fablics tots els detalls dels estudis posteriors, per aix\u00ed seguir contribuint en la col\u00b7laboraci\u00f3 i l\u2019aven\u00e7 del camp.<\/p>\n

 <\/p>\n

Els dos m\u00e8todes de seq\u00fcenciaci\u00f3<\/h4>\n

 <\/p>\n

En l\u2019estudi s\u2019han comparat dos m\u00e8todes de seq\u00fcenciaci\u00f3: el 16s i el Shotgun. El primer desxifra la seq\u00fc\u00e8ncia d\u2019un sol gen dels microorganismes, mentre que el segon ens dona la seq\u00fc\u00e8ncia completa de tot el genoma. Tot i que en seq\u00fcenciar un \u00fanic gen perdem resoluci\u00f3, pot resultar m\u00e9s econ\u00f2mic. A m\u00e9s, el fet de seq\u00fcenciar un gen nom\u00e9s present en la microbiota ens permet analitzar mostres de bi\u00f2psies sense que el genoma hum\u00e0 hi interfereixi.<\/p>\n

Addicionalment, la prova pilot ha mostrat que ambdues t\u00e8cniques s\u00f3n consistents. Tot i que la seq\u00fcenciaci\u00f3 completa \u00e9s m\u00e9s sensible i hi distingim m\u00e9s esp\u00e8cies de microorganismes, en cap moment contradiu els resultats obtinguts en seq\u00fcenciar un \u00fanic gen.<\/p>\n","protected":false},"excerpt":{"rendered":"

En aquest estudi pilot s\u2019han comparat dos m\u00e8todes de seq\u00fcenciaci\u00f3, utilitzant mostres de bi\u00f2psies de c\u00f2lon i mostres de femta de nou pacients. Tamb\u00e9 s\u2019han definit els m\u00e8todes d\u2019an\u00e0lisi bioinform\u00e0tic m\u00e9s adients per a aquest tipus d\u2019estudis.<\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[332,375],"tags":[],"class_list":["post-7319","post","type-post","status-publish","format-standard","hentry","category-cancer","category-colorectal"],"publishpress_future_action":{"enabled":false,"date":"2025-01-29 08:01:48","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7319","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/comments?post=7319"}],"version-history":[{"count":0,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/posts\/7319\/revisions"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media?parent=7319"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/categories?post=7319"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/tags?post=7319"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}