{"id":973,"date":"2019-05-23T05:13:17","date_gmt":"2019-05-23T03:13:17","guid":{"rendered":"https:\/\/idibell.cat\/?page_id=973"},"modified":"2023-03-09T17:59:26","modified_gmt":"2023-03-09T16:59:26","slug":"citometria-de-flux","status":"publish","type":"page","link":"https:\/\/idibell.cat\/serveis\/serveis-cientificotecnics\/citometria-de-flux\/","title":{"rendered":"Citometria de flux"},"content":{"rendered":"\n<h1>\n\t\tCitometria de flux\n\t<\/h1>\n\tLa nostra plataforma de citometria de flux proporciona als usuaris equipament d&#8217;\u00faltima generaci\u00f3 per a les an\u00e0lisis de citometria de flux i la classificaci\u00f3 de c\u00e8l\u00b7lules en alta velocitat. El personal de la unitat dona suport als usuaris en el disseny experimental, l&#8217;an\u00e0lisi i l&#8217;adquisici\u00f3 de dades.<br \/>\nSeguint les instruccions de fabricant, els equips se sotmeten rutin\u00e0riament a un manteniment adequat i es duen a terme procediments de control de qualitat est\u00e0ndard per garantir els millors resultats.\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/uploads\/2023\/03\/FACS.jpg\" alt=\"FACS\" itemprop=\"image\" height=\"290\" width=\"515\" title=\"FACS\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-0\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-0\">Serveis<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-0\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-0\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<p>Serveis:<\/p>\n<ul>\n<li>Adquisici\u00f3 de mostres a l&#8217;analitzador, ja sigui amb suport t\u00e8cnic o sense, despr\u00e9s de rebre una formaci\u00f3 adequada.<\/li>\n<li>Classificaci\u00f3 cel\u00b7lular amb suport t\u00e8cnic.<\/li>\n<li>Suport per a l&#8217;an\u00e0lisi de dades si \u00e9s necessari.<\/li>\n<\/ul>\n<p>L&#8217;objectiu de la Unitat de Citometria \u00e9s preparar-se i centrar-se en les aplicacions d&#8217;inter\u00e8s dels nostres usuaris. Les aplicacions m\u00e9s comuns que s&#8217;executen habitualment s\u00f3n:<\/p>\n<p><strong>Classificaci\u00f3 cel\u00b7lular:<\/strong><\/p>\n<ul>\n<li>En  <strong>mode de purificaci\u00f3<\/strong> (quan el m\u00e9s important \u00e9s obtenir una alta puresa de la poblaci\u00f3 cel\u00b7lular d&#8217;inter\u00e8s de la mostra original). Fins a 4 poblacions de c\u00e8l\u00b7lules al mateix temps (classificaci\u00f3 de 4 vies).<\/li>\n<li>En <strong>mode enriquit <\/strong>(quan el m\u00e9s important \u00e9s obtenir una alta recuperaci\u00f3 de la poblaci\u00f3 cel\u00b7lular d&#8217;inter\u00e8s de la mostra original). De manera que la puresa es veu lleugerament compromesa. Tamb\u00e9 disponible en classificaci\u00f3 de 4 direccions.<\/li>\n<li>En d<strong>eposici\u00f3 d&#8217;una sola c\u00e8l\u00b7lula<\/strong> (per obtenir una sola c\u00e8l\u00b7lula o qualsevol nombre desitjat de c\u00e8l\u00b7lula dins d&#8217;un sol pouet utilitzant qualsevol de les plaques est\u00e0ndard de m\u00faltiples orificis).<\/li>\n<li>En <strong>mode mixt<\/strong> (el mode de purificaci\u00f3 i enriquiment s&#8217;executen simult\u00e0niament fins i tot per a 2 poblacions de c\u00e8l\u00b7lules alhora).<\/li>\n<\/ul>\n<p><strong>An\u00e0lisi:<\/strong><\/p>\n<ul>\n<li>Caracteritzaci\u00f3 per inmunofenotipado multicolor.<\/li>\n<li>Assaigs de viabilitat.<\/li>\n<li>Assaigs de cicle cel\u00b7lular i proliferaci\u00f3.<\/li>\n<li>Expressi\u00f3 g\u00e8nica (an\u00e0lisi de prote\u00efnes fluorescents).<\/li>\n<li>Etc ..<\/li>\n<\/ul>\n<p>.<\/p>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-1\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-1\">Aparells<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-1\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-1\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<p>La unidad est\u00e0 equipada amb 3 cit\u00f2metres de flux de la companyia Beckman Coulter:<\/p>\n<ul>\n<li>1x classificador de c\u00e8l\u00b7lules d&#8217;alta velocitat Moflo-XDP: 4 l\u00e0sers (B-R-V-Y), 11 colors (5 + 2 + 2 + 2) o (5 + 2 + 4)<\/li>\n<li>Analitzadors 2x Galios: 3 l\u00e0sers (B-R-V), 10 colors (5 + 3 + 2).<\/li>\n<\/ul>\n<br \/>\n<em>Abreviatures de\u00e0ser i configuraci\u00f3 del banc \u00f2ptic:\n&nbsp;&nbsp;&nbsp;L\u00e0sers:<br \/>\n&nbsp;&nbsp;&nbsp; V: violeta, 405 nm; B:blau, 488 nm; Y: groc, 561 nm; R: vermell, 635 nm.\n&nbsp;&nbsp;&nbsp;Configuraci\u00f3 de banc \u00f2ptic:<br \/>\n&nbsp;&nbsp;&nbsp;10C [2 + 5 + 3] = 10 colors o detectors PMT en total i [nombre d&#8217;ells vinculats a una l\u00ednia de l\u00e0ser espec\u00edfica).<\/em>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-2\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-2\">Publicacions<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-2\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-2\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<p>Bedford-Guaus S, Kim S, Mulero L, <strong>Vaquero JM<\/strong>, Morera C, Adan-Milan\u00e8s R, Veiga A, Raya A. (2016)<strong> Molecular markers of putative spermatogonial stem cells in the domestic cat. Reprod Domest Anim Nov6<\/strong><\/p>\n<p>Pulecio J, Alejo-Valle O, Capellera-Garcia S, Vitaloni M, Rio P, Mej\u00eda-Ram\u00edrez E, Caserta I, Bueren JA, Flygare J, Raya A. (2016) <strong>Direct Conversion of Fibroblasts to Megakaryocyte Progenitors. Cell Rep 17(3):671-683.<\/strong><\/p>\n<p>Tiscornia G, Lorenzo Vivas E, Matalonga L, Berniakovich I, Barrag\u00e1n Monasterio M, Eguiz\u00e1bal Argaiz C, Gort L, Gonz\u00e1lez F, Mellet CO, Garc\u00eda Fern\u00e1ndez JM, Ribes A, Veiga A, Izpis\u00faa Belmonte JC. (2013)<strong> Neuronopathic Gaucher&#8217;s Disease: induced pluripotent stem cells for disease modelling and testing chaperone activity of small compounds. Hum Mol Genet 22 (4): 633-45. Epub 2012 Oct 31.<\/strong><\/p>\n<p>Sander V, Su\u00f1e G, Jopling C, Morera C, Izpisua Belmonte JC. (2013)<strong> Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts. Nat Protoc 8(4): 800-9.<\/strong><\/p>\n<p>Marti M, Montserrat N, Pardo C, Mulero L, Miquel-Serra L, Rodrigues AM, <strong>Andr\u00e9s Vaquero J<\/strong>, Kuebler B, Morera C, Barrero MJ, Izpisua Belmonte JC. (2013) <strong>Mcad-mediated intercellular interactions activate satellite cell division. J Cell Sci 126 (Pt 22):5116-31. Epub 2013 Sep 17.<\/strong><\/p>\n<p>Barrero MJ, Sese B, Mart\u00ed M, Izpis\u00faa Belmonte JC. (2013) <strong>Macro histone variants are critical for the differentiation of human pluripotent cells. J Biol Chem 288(22):16110-6. Epub 2013 Apr 17.<\/strong><\/p>\n<p>Montserrat N, de O\u00f1ate L, Garreta E, et al. <strong>Generation of feeder-free pig induced pluripotent stem cells without Pou5f1. Cell Transplant. 2012;21(5):815-825<\/strong><\/p>\n<p>Montserrat N, Ram\u00edrez-Bajo MJ, Xia Y, et al. <strong>Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2. J Biol Chem. 2012;287(29):24131-24138.<\/strong><\/p>\n<p>Giorgetti A, Marchetto MC, Li M, et al. <strong>Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc. Proc Natl Acad Sci U S A. 2012;109(31):12556-12561.<\/strong><\/p>\n<p>S\u00e1nchez-Dan\u00e9s A, Consiglio A, Richaud Y, et al. <strong>Efficient generation of A9 midbrain dopaminergic neurons by lentiviral delivery of LMX1A in human embryonic stem cells and induced pluripotent stem cells. Hum Gene Ther. 2012;23(1):56-69.<\/strong><\/p>\n<p>Robles V, Mart\u00ed M, Izpisua Belmonte JC. <strong>Study of pluripotency markers in zebrafish embryos and transient embryonic stem cell cultures. Zebrafish. 2011;8(2):57-63<\/strong><\/p>\n<p>Eguizabal C, Montserrat N, Vassena R, et al. <strong>Complete meiosis from human induced pluripotent stem cells. Stem Cells. 2011;29(8):1186-1195.<\/strong><\/p>\n<p>Mench\u00f3n C, Edel MJ, Izpisua Belmonte JC. <strong>The cell cycle inhibitor p27Kip\u00b9 controls self-renewal and pluripotency of human embryonic stem cells by regulating the cell cycle, Brachyury and Twist. Cell Cycle. 2011;10(9):1435-1447.<\/strong><\/p>\n<p>Edel MJ, Menchon C, <strong>Vaquero JM<\/strong>, Izpis\u00faa Belmonte JC. (2011) <strong>A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells. Cell Commun Signal 9(1): 8.<\/strong><\/p>\n<p>Edel MJ, Menchon C, Menendez S, Consiglio A, Raya A, Izpisua Belmonte JC. <strong>Rem2 GTPase maintains survival of human embryonic stem cells as well as enhancing reprogramming by regulating p53 and cyclin D1. Genes Dev. 2010;24(6):561-573.<\/strong><\/p>\n<p>Laso FJ, Almeida J, Torres E, <strong>Vaquero JM<\/strong>, Marcos M, Orfao A. (2010) <strong>Chronic alcohol consumption is associated with an increased cytotoxic profile of circulating lymphocytes that may be related with the development of liver injury. Alcohol Clin Exp Res 34(5): 876-85.<\/strong><\/p>\n<p>Raya A, Rodr\u00edguez-Piz\u00e0 I, Guenechea G, et al. <strong>Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature. 2009;460(7251):53-59.<\/strong><\/p>\n<p>Aasen T, Raya A, Barrero MJ, et al. <strong>Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008;26(11):1276-1284.<\/strong><\/p>\n<p>Laso FJ, <strong>Vaquero JM<\/strong>, Almeida J, Marcos M and Orfao A. (2007) <strong>Chronic alcohol consumption is associated with changes in the distribution, immunophenotype, and the inflammatory cytokine secretion profile of circulating dendritic cells. Alcoholism Clinical and Experimental Research 31(5): 846-854.<\/strong><\/p>\n<p>Laso FJ, <strong>Vaquero JM<\/strong>, Almeida J, Marcos M and Orfao A. (2007)<strong> Production of inflammatory cytokines by peripheral blood monocytes in chronic alcoholism: Relationship with ethanol intake and liver disease. Cytometry Part B Clinical Cytometry 72(5): 408-15.<\/strong><\/p>\n<p>Almeida J, Bueno C, Alguer\u00f3 MC, S\u00e1nchez ML, Santiago M, Escribano L, Diaz-Agustin B, <strong>Vaquero JM<\/strong>, Laso FJ, San Miguel JF, and Orfao A. (2001)<strong> Comparative Analysis of the Morphological, Cytochemical, Immunophenotypical, and Functional Characteristics of Normal Human Peripheral Blood Lineage- \/ CD16+ \/ HLA-DR+ \/ CD14-\/lo Cells, CD14+ \/ Monocytes, and CD16- Dendritic Cells. Clinical Immunology 100(3): 325-338.<\/strong><\/p>\n<p>Bueno C, Almeida J, Alguer\u00f3 MC, S\u00e1nchez ML, <strong>Vaquero JM<\/strong>, Laso FJ, San Miguel JF, Escribano L, and Orfao A. (2001) <strong>Flow Cytometric Analysis of Cytokine Production by Normal Human Peripheral Blood Dendritic Cells and Monocytes: Comparative Analysis of Different Stimuli, Secretion-Blocking Agents and Incubation Periods. Cytometry 46: 33-40.<\/strong><\/p>\n<p>Bueno C, Almeida J, Alguer\u00f3 MC, S\u00e1nchez ML, Ca\u00f1izo MC, Fern\u00e1ndez ME, <strong>Vaquero JM<\/strong>, Laso FJ, Escribano L, D\u00edaz-Agustin B, San Miguel JF, y Orfao A. (2000) <strong>Caracterizaci\u00f3n morfol\u00f3gica, histoqu\u00edmica, fenot\u00edpica y funcional de las c\u00e9lulas dendr\u00edticas humanas. Methods Find Exp Clin Pharmacol 22(1): 3-10.<\/strong><\/p>\n<p>Laso FJ, <strong>Vaquero JM<\/strong>, Bueno C, Almeida J, and Orfao A. (2000) <strong>Response. Alcoholism: Clinical and Experimental Research 24(2): 241-243.<\/strong><\/p>\n<p>Almeida J, Bueno C, Alguer\u00f3 MC, S\u00e1nchez ML, Ca\u00f1izo MC, Fern\u00e1ndez ME, <strong>Vaquero JM<\/strong>, Laso FJ, Escribano L, San Miguel JF, and Orfao A. (1999) <strong>Extensive characterization of the imnuphenotype and pattern of citokine production by distinct subpopulations of normal human peripheral blood MHC II+\/lineage- cells. Clin Exp Immunol 118: 392-401.<\/strong><\/p>\n<p>Almeida J, Bueno C, Alguer\u00f3 MC, S\u00e1nchez ML, Ca\u00f1izo MC, Fern\u00e1ndez ME, <strong>Vaquero JM<\/strong>, Laso FJ, Escribano L, San Miguel JF, y Orfao A. (1999)<strong> Subpoblaciones de c\u00e9lulas dendr\u00edticas normales: fenotipo y patr\u00f3n de secreci\u00f3n de citocinas. Haematologica 84(1): 408-414.<\/strong><\/p>\n<p>Almeida J, Bueno C, Alguer\u00f3 MC, S\u00e1nchez ML, <strong>Vaquero JM<\/strong>, San Miguel JF, and Orfao A. (1999) <strong>Imnunophenotype and citokine production by normal human peripheral blood dendritic cell subsets. Haematologica vol. 84, EHA-4 Abstract Book: 136.<\/strong><\/p>\n\t<p><div class=\"ast-oembed-container \" style=\"height: 100%;\"><iframe loading=\"lazy\" title=\"Els serveis cientificot\u00e8cnics de l&#039;IDIBELL\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/ZaXrvEbwE5U?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/div><\/p>\n<p>&nbsp;<\/p>\n<p>Descobreix els serveis cientificot\u00e8cnics de l&#8217;IDIBELL!<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/uploads\/2022\/02\/IMG_6627-scaled-e1644487233947-150x150.jpg\" alt=\"IMG_6627\" itemprop=\"image\" height=\"150\" width=\"150\" title=\"IMG_6627\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\tJos\u00e9 Miguel Andr\u00e9s Vaquero\n\t<\/h4>\n<h4>\n\t\tCONTACTA\n\t<\/h4>\n<h4>\n\t\t<a href=\"mailto:flowcytometry@idibell.cat\" title=\"Citometria de flux\" target=\"_blank\" rel=\"noopener\">\n\t\tCitometria de flux\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\tDOCUMENTS\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/drive.google.com\/file\/d\/1O0FpRa5gOhHjnDgph4Br8zIhFi9-G9Ad\/view?usp=sharing\" title=\"Tarifes Plataformes [pdf]\" target=\"_blank\" rel=\"noopener\">\n\t\tTarifes Plataformes [pdf]\n\t\t<\/a>\n\t<\/h4>\n<h2>\n\t\tEquip\n\t<\/h2>\n\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-tabs--label-0\" data-index=\"0\" aria-selected=\"true\" aria-controls=\"fl-tabs--panel-0\" aria-expanded=\"true\" role=\"tab\" tabindex=\"0\">\t\t\t\tCap de Plataforma\t\t\t\t<\/a>\n\t\t\t\t\t\tCap de Plataforma\n\t\t\t\t\t\t\t\t\t\t\t<ul>\n<li>Jos\u00e9 Miguel Andr\u00e9s Vaquero<\/li>\n<\/ul>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\n","protected":false},"excerpt":{"rendered":"<p>Citometria de flux La nostra plataforma de citometria de flux proporciona als usuaris equipament d&#8217;\u00faltima generaci\u00f3 per a les an\u00e0lisis de citometria de flux i la classificaci\u00f3 de c\u00e8l\u00b7lules en alta velocitat. El personal de la unitat dona suport als usuaris en el disseny experimental, l&#8217;an\u00e0lisi i l&#8217;adquisici\u00f3 de dades. Seguint les instruccions de fabricant, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":679,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"publishpress_future_action":{"enabled":false,"date":"2024-05-11 10:41:09","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/973"}],"collection":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/comments?post=973"}],"version-history":[{"count":5,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/973\/revisions"}],"predecessor-version":[{"id":23255,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/973\/revisions\/23255"}],"up":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/679"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media?parent=973"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}