{"id":764,"date":"2019-05-15T08:47:41","date_gmt":"2019-05-15T06:47:41","guid":{"rendered":"https:\/\/idibell.cat\/?page_id=764"},"modified":"2023-09-01T14:36:46","modified_gmt":"2023-09-01T12:36:46","slug":"interaccions-moleculars","status":"publish","type":"page","link":"https:\/\/idibell.cat\/serveis\/serveis-cientificotecnics\/interaccions-moleculars\/","title":{"rendered":"Interaccions Moleculars"},"content":{"rendered":"\n<h1>\n\t\tInteraccions Moleculars\n\t<\/h1>\n\tLa Plataforma d&#8217;Interaccions Moleculars proporciona suport cient\u00edfic i tecnol\u00f2gic a la comunitat cient\u00edfica en el camp de les interaccions moleculars i el metabolisme utilitzant diversos abordatges. En primer lloc, Surface Plasmon Resonance (SPR) permet la monitoritzaci\u00f3 en temps real de qualsevol tipus d&#8217;interaccions moleculars (prote\u00efna-prote\u00efna, prote\u00efna-f\u00e0rmacs, prote\u00efna-ves\u00edcules, prote\u00efna-RNA, &#8230;) i realitza mesures d&#8217;alta precisi\u00f3 de les constants d&#8217;afinitat i cin\u00e8tiques, l&#8217;especificitat d&#8217;uni\u00f3, isotipatge i cribratge de possibles medicaments \/ f\u00e0rmacs. A continuaci\u00f3, les tecnologies Luminex i SIMOA permeten la detecci\u00f3 de fins a cinquanta biomarcadors diferents alhora en mostres biol\u00f2giques i fluids utilitzant la tecnologia de perles magn\u00e8tiques. Finalment, el metabolisme i la bioenerg\u00e8tica, que controlen funcions cel\u00b7lulars com la senyalitzaci\u00f3 cel\u00b7lular, la proliferaci\u00f3 o el dest\u00ed cel\u00b7lular, poden ser mesurats mitjan\u00e7ant el Seahorse en temps real en c\u00e8l\u00b7lules vives. La respiraci\u00f3 mitocondrial, la gluc\u00f2lisi, la taxa d&#8217;oxidaci\u00f3 d&#8217;ATP i l&#8217;oxidaci\u00f3 d&#8217;\u00e0cids grassos es poden mesurar amb alta precisi\u00f3.<br \/>\nLa nostra missi\u00f3 \u00e9s oferir un suport integrat, des del disseny experimental, l&#8217;optimitzaci\u00f3 de les condicions de l&#8217;assaig, l&#8217;execuci\u00f3 d&#8217;experiments fins a l&#8217;an\u00e0lisi de dades i la generaci\u00f3 d&#8217;informes. La nostra tecnologia punta juntament amb la gran experi\u00e8ncia i coneixement en recerca biom\u00e8dica i ci\u00e8ncia de prote\u00efnes estan alservei d&#8217;investigadors interns i externs.\n\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/uploads\/2023\/03\/Molec-Interactions-2-1.jpg\" alt=\"Molec-Interactions-2-1\" itemprop=\"image\" height=\"383\" width=\"584\" title=\"Molec-Interactions-2-1\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-0\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-0\">Serveis<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-0\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-0\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<ul>\n<li>Determinaci\u00f3 de la constant d&#8217;afinitat (KD), cin\u00e8tica: constants d&#8217;associaci\u00f3 i dissociaci\u00f3 (kon, Koff) en diferents models (prote\u00efna-prote\u00efna, prote\u00efna-p\u00e8ptid, prote\u00efna-mol\u00e8cula petita, prote\u00efna-RNA &#8230;)<\/li>\n<li>Determinaci\u00f3 de la concentraci\u00f3 efectiva i control de qualitat<\/li>\n<li>Isotipado d&#8217;anticossos<\/li>\n<li>Cribratge de possibles f\u00e0rmacs \/ biosimilars<\/li>\n<li>Quantificaci\u00f3 de fins a 50 biomarcadors en mostres i fluids biol\u00f2gics sobre planxa de 96 pous per Luminex<\/li>\n<li>Quantificaci\u00f3 ultra-sensitiva de fins a 4 biomarcadors en mostres i fluids biol\u00f2gics sobre planxa de 96 pous per SIMOA<\/li>\n<li>Mesura de la taxa de consum d&#8217;oxigen (OCR) i la taxa d&#8217;acidificaci\u00f3 extracel\u00b7lular (ECAR) sobre planxa de 96 pous (Seahorse): respiraci\u00f3 mitocondrial, gluc\u00f2lisi, producci\u00f3 d&#8217;ATP, lip\u00f2lisi<br \/>\nEscaneig fluorescent de diversos formats<\/li>\n<li>Configuraci\u00f3 i optimitzaci\u00f3 de condicions experimentals<\/li>\n<li>Suport cientificotecnol\u00f2gic a investigadors<\/li>\n<li>Capacitaci\u00f3 de nous usuaris en l&#8217;\u00fas de l&#8217;equip.<\/li>\n<\/ul>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-1\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-1\">Aparells<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-1\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-1\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<ul>\n<li>Biacore T-200 (GE) per l&#8217;an\u00e0lisi SPR d&#8217;interaccions<\/li>\n<li>Analitzador Seahorse XFe96 (Agilent) per a la detecci\u00f3 de OCR i ECAR en una placa de 96 pouets<\/li>\n<li>Magpix (Luminex) que inclou programari xPonent 4.2 per a an\u00e0lisi m\u00faltiplex de fins a 50 perles magn\u00e8tiques<\/li>\n<li>Sistema de detecci\u00f3 de biomarcadors ultra-sensitiu SIMOA SR-X (Quanterix)<\/li>\n<li>Esc\u00e0ner de fluoresc\u00e8ncia Typhoon FLA9500 (GE) equipat amb l\u00e0ser blau, verd i vermell<\/li>\n<li>Programari ImageQuant TL 7.0<\/li>\n<\/ul>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-2\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-2\">Publicacions<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-2\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-2\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<p>The NADPH oxidase NOX4 regulates redox and metabolic homeostasis preventing HCC progression. Pe\u00f1uelas-Haro, I., Espinosa-Sotelo, R., Crosas-Molist, E., &#8230; Bertran, E. and Fabregat, I.\u00a0Hepatology 2023, 78, 416-433. (agra\u00efments Seahorse)<\/p>\n<p>A novel family of lysosomotropic tetracyclic compounds for treating leukemia. Carb\u00f3, J.M., Cornet-Masana, J.M., .. and Risue\u00f1o, R.M. Cancers 2023, 15, 1912 (agra\u00efments Seahorse)<\/p>\n<p>Dual and opposite costimulatory targeting with a novel human fusion recombinant protein effectively prevents renal warm ischemia reperfusion injury and allograft rejection in murine models. Guiteras, J, De Ramon, L., Crespo, E, Bola\u00f1os, N, <strong>Barcel\u00f3-Batllori, S<\/strong>., et al. International Journal of Molecular Sciences, 2021, 22, 1216<\/p>\n<p>Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis. Colom\u00e9, N., Abi\u00e1n, J., Aloria, K., Arizmendi, JM., <strong>Barcel\u00f3-Batllori, S<\/strong>., et al. Journal of Proteomics, 2022, 251, 104409<\/p>\n<p>Urinary cytokines reflect renal inflammation in acute tubulointerstitial nephritis: A multiplex bead-based assay assessment. Martinez Valenzulea, L., Draibe, J., Bestard, O., Fulladosa, X., et al. Journal of Clinical Medicine, 2021, 10, 2986 (agra\u00efments Luminex)<\/p>\n<p>Immunodominant proteins P1 and P40\/P90 from human pathogen Mycoplasma pneumoniae. Vizarraga, D., Kawamoto, A., Matsumoto J, Illanes, R., P\u00e9rez-Luque, R., et al. Nature Communications, 2020, 11, 5188 (agra\u00efments Biacore)<\/p>\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29317652\/\" target=\"_blank\" rel=\"noopener\">SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-kB pathway. <\/a><br \/>\nSantos-Barriopedro, I., Bosch-Presegu\u00e9, L., Marazuela-Duque, A., de la Torre, C., Colomer, C., Vazquez, B.N., Fuhrmann, T., Mart\u00ednez-Pastor, B., Lu, W., Braun, T., Bober, E., Jenuwein, T., Serrano, L., Esteller, M., Cheng, Z., <strong>Barcel\u00f3-Batllori, S<\/strong>., Mostoslavsky, R., Espinosa, Ll., &amp; Vaquero, A.<br \/>\nNature communications, 2018, 9:101. doi: 10.1038\/s41467-017-02586\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26208904\/\" target=\"_blank\" rel=\"noopener\">KAT6B is a tumor suppressor histone H3 lysine 23 acetyltransferase undergoing genomic loss in small cell lung cancer.<\/a><br \/>\nSim\u00f3-Riudalbas, L., P\u00e9rez-Salvia, M., Setien F., Villanueva, A., Moutinho, C., Mart\u00ednez-Card\u00fas, A., Moran, S., Berdasco, M., Gomez, A., Vidal, E., Soler, M., Heyn, H., Vaquero, A., de la Torre, C.,<strong> Barcel\u00f3-Batllori <\/strong>S, &#8230; et Esteller, M<br \/>\nCancer Research 2015, 75, 3936-3945\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/17986440\/\" target=\"_blank\" rel=\"noopener\">Integration of DIGE and bioinformatics analyses reveals a role of the anti-obesity agent tungstate in redox and energy homeostasis pathways in brown adipose tissue.<\/a><br \/>\n<strong>Barcel\u00f3-Batllori, S<\/strong>., Esteban, Y. Moreno, S., Kalko, S. and Gomis, R.<br \/>\nMolecular and Cellular Proteomics, 2008, 7, 378-393\n\t<p><div class=\"ast-oembed-container \" style=\"height: 100%;\"><iframe loading=\"lazy\" title=\"Els serveis cientificot\u00e8cnics de l&#039;IDIBELL\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/ZaXrvEbwE5U?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/div><\/p>\n<p>&nbsp;<\/p>\n<p>Descobreix els serveis cientificot\u00e8cnics de l&#8217;IDIBELL!<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/uploads\/2022\/02\/IMG_6653-scaled-e1644488127612-150x150.jpg\" alt=\"IMG_6653\" itemprop=\"image\" height=\"150\" width=\"150\" title=\"IMG_6653\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\tSilvia Barcel\u00f3\n\t<\/h4>\n<h4>\n\t\tCONTACTA\n\t<\/h4>\n<h4>\n\t\t<a href=\"mailto:molecularinteractions@idibell.cat\" title=\"Interaccions Moleculars\" target=\"_blank\" rel=\"noopener\">\n\t\tInteraccions Moleculars\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\tDOCUMENTS\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/drive.google.com\/file\/d\/1O0FpRa5gOhHjnDgph4Br8zIhFi9-G9Ad\/view?usp=sharing\" title=\"Tarifes Plataformes [pdf]\" target=\"_blank\" rel=\"noopener\">\n\t\tTarifes Plataformes [pdf]\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/wp-content\/uploads\/2020\/11\/SCT-MI-FOR-01001ENG-Request-Biacore-v1.docx\" title=\"Full Sol\u00b7licitud Biacore\" target=\"_blank\" rel=\"noopener\">\n\t\tFull Sol\u00b7licitud Biacore\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/wp-content\/uploads\/2020\/11\/SCT-MI-FOR-01003ENG-Request-Luminex-v1.docx\" title=\"Full Sol\u00b7licitud Luminex\" target=\"_blank\" rel=\"noopener\">\n\t\tFull Sol\u00b7licitud Luminex\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/wp-content\/uploads\/2020\/11\/SCT-MI-FOR-01004ENG-Request-Seahorse.docx\" title=\"Full Sol\u00b7licitud Seahorse\" target=\"_blank\" rel=\"noopener\">\n\t\tFull Sol\u00b7licitud Seahorse\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/wp-content\/uploads\/2020\/11\/SCT-MI-FOR-01005ENG-Request-Typhoon.docx\" title=\"Full Sol\u00b7licitud Typhoon\" target=\"_blank\" rel=\"noopener\">\n\t\tFull Sol\u00b7licitud Typhoon\n\t\t<\/a>\n\t<\/h4>\n<h2>\n\t\tEquip\n\t<\/h2>\n\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-tabs--label-0\" data-index=\"0\" aria-selected=\"true\" aria-controls=\"fl-tabs--panel-0\" aria-expanded=\"true\" role=\"tab\" tabindex=\"0\">\t\t\t\tCap de Plataforma\t\t\t\t<\/a>\n\t\t\t\t\t\tCap de Plataforma\n\t\t\t\t\t\t\t\t\t\t\t<ul>\n<li>Silvia Barcel\u00f3 Batllori<\/li>\n<\/ul>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\n","protected":false},"excerpt":{"rendered":"<p>Interaccions Moleculars La Plataforma d&#8217;Interaccions Moleculars proporciona suport cient\u00edfic i tecnol\u00f2gic a la comunitat cient\u00edfica en el camp de les interaccions moleculars i el metabolisme utilitzant diversos abordatges. En primer lloc, Surface Plasmon Resonance (SPR) permet la monitoritzaci\u00f3 en temps real de qualsevol tipus d&#8217;interaccions moleculars (prote\u00efna-prote\u00efna, prote\u00efna-f\u00e0rmacs, prote\u00efna-ves\u00edcules, prote\u00efna-RNA, &#8230;) i realitza mesures d&#8217;alta [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":679,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"unboxed","site-sidebar-style":"unboxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-764","page","type-page","status-publish","hentry"],"publishpress_future_action":{"enabled":false,"date":"2024-11-25 01:16:56","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/comments?post=764"}],"version-history":[{"count":5,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/764\/revisions"}],"predecessor-version":[{"id":24160,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/764\/revisions\/24160"}],"up":[{"embeddable":true,"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/pages\/679"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/wp-json\/wp\/v2\/media?parent=764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}