Regenerative medicine for the treatment of heart disease
Problema a solucionar
During last decades, much effort has been directed toward using stem cell therapy for cardiac regeneration. Although it has been demonstrated to be feasible and safe in animal models, the results have not been the same for patients. Heart transplant is the only therapy capable of restoring heart function after Acute Myocardial Infarction. However, transplantation is dependent on the number of donors and rejection reactions. Thus, there is an urgent need to find an alternative useful therapy
One application for this technology is the in vitro use of a cardiac construct for the screening or evaluation of compounds for cardioprotective or cardiotoxic properties, and for cardiac disease modeling.
The present invention also represents a new therapeutic option for patients with acute myocardial infarction, which present post-infarct scars. CTE products could be implanted in damaged myocardium and shrink simultaneously with healthy tissue. At that moment, an improvement in contractile strength would occur, as well as in patient’s life quality and life expectancy.
Estat de la tecnologia
Preclinical studies have been completed, demonstrating the efficacy in reproducing cardiomyocytes. Future work: CTE products, generated under GMP conditions and following AEMPS policies, which consist of 2 elements: decellularized scaffolds (biocompatible and highly similar to native myocardium) and cellular component (Wharton’s Jelly-derived Mesenchymal Stem Cells or iPS). Preclinical studies have to be completed, demonstrating the security and efficacy of CTE products (in vitro and in vivo – mice and pork). It is expected to reduce infarct size and to improve the functionality. A pilot study is being prepared to be performed in patients. IP status: Patent application in June 2018.
The main problems using stem cell therapy in cardiac regeneration include: the big surface of the scar to be repaired, the low retention degree of administered cells and the limited survival of implanted cells. For this reason, therapeutic efforts are being refocused on Cardiac Tissue Engineering (CTE), which uses scaffolds (either biological or synthetic) and stem cells, directly injected into the infarct zone. Although researchers are obtaining good preclinical results, these are not being translated to clinical assays. One of the critical points to generate a functional myocardium is the use of scaffolds with high similarity to the native myocardium. For this reason, the authors of the presented technology use decellularized scaffolds from heart biopsies. Another common problem in transplants is the reaction of immune response. To avoid it, the authors have used multipotent mesenchymal stem cells (because of their regenerative and immunomodulatory activity) and iPS (reprogramming somatic cells towards pluripotency by defined factors).
The authors have developed two exploitable technologies: 1. A system that allows the generation of mature human cardiomyocytes. The technology is based on bioreactors that enable 3D culture of patient-derived-iPS. 2. A cardiopatch recovered by cardiomyocytes that could be used for human therapies in patients with heart damage.
Raya A (IDIBELL)
Technology Readiness Level
Acord de llicència