{"id":764,"date":"2019-05-15T08:47:41","date_gmt":"2019-05-15T08:47:41","guid":{"rendered":"https:\/\/idibell.cat\/?page_id=764"},"modified":"2025-03-07T08:57:03","modified_gmt":"2025-03-07T07:57:03","slug":"interacciones-moleculares","status":"publish","type":"page","link":"https:\/\/idibell.cat\/es\/servicios\/servicios-cientificotecnicos\/interacciones-moleculares\/","title":{"rendered":"Interacciones Moleculares"},"content":{"rendered":"\n<h1>\n\t\tInteracciones Moleculares\n\t<\/h1>\n\tLa Plataforma de Interacciones Moleculares proporciona apoyo cient\u00edfico-tecnol\u00f3gico a la comunidad cient\u00edfica en el campo de las interacciones moleculares y el descubrimiento de biomarcadores utilizando varios enfoques. En primer lugar, Surface Plasmon Resonance (SPR) permite la monitorizaci\u00f3n en tiempo real de cualquier tipo de interacciones moleculares (prote\u00edna-prote\u00edna, prote\u00edna-f\u00e1rmacos, prote\u00edna-ves\u00edculas, prote\u00edna-ARN,&#8230;) y realiza mediciones de alta precisi\u00f3n de las constantes de afinidad y cin\u00e9ticas, la especificidad de uni\u00f3n, isotipado y cribado de posibles medicamentos \/ f\u00e1rmacos. A continuaci\u00f3n, las tecnolog\u00edas Luminex y SIMOA permiten la detecci\u00f3n de hasta cincuenta biomarcadores diferentes a la vez en muestras biol\u00f3gicas y fluidos utilizando la tecnolog\u00eda de perlas magn\u00e9ticas. Por \u00faltimo, el metabolismo y la bioenerg\u00e9tica, que controlan funciones celulares como la se\u00f1alizaci\u00f3n celular, la proliferaci\u00f3n o el destino celular, pueden ser medidos mediante el Seahorse en tiempo real en c\u00e9lulas vivas. La respiraci\u00f3n mitocondrial, la gluc\u00f3lisis, la tasa de oxidaci\u00f3n de ATP y la oxidaci\u00f3n de \u00e1cidos grasos se pueden medir con alta precisi\u00f3n.<br \/>\nRecientemente hemos adquirido una plataforma de transcript\u00f3mica y prote\u00f3mica espacial de alta resoluci\u00f3n, CosMx (Nanostring). CosMx SMI permite la r\u00e1pida cuantificaci\u00f3n y visualizaci\u00f3n de hasta 6000 ARN y 64 analitos proteicos validados con resoluci\u00f3n de c\u00e9lula \u00fanica en biopsias y muestras de tejidos.<br \/>\nNuestra misi\u00f3n es ofrecer un soporte integrado, desde el dise\u00f1o experimental, la optimizaci\u00f3n de las condiciones del ensayo, la ejecuci\u00f3n de experimentos hasta el an\u00e1lisis de datos y la generaci\u00f3n de informes. Nuestra tecnolog\u00eda punta junto con la gran experiencia y conocimiento en investigaci\u00f3n biom\u00e9dica y ciencia de prote\u00ednas est\u00e1n al servicio de investigadores internos y externos.\n\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/uploads\/sites\/4\/2023\/03\/Molec-Interactions-2-1.jpg\" alt=\"Molec-Interactions-2-1\" itemprop=\"image\" height=\"383\" width=\"584\" title=\"Molec-Interactions-2-1\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-0\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-0\">Servicios<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-0\" tabindex=\"-1\"><i title=\"Expand\">Expand<\/i><\/a>\n\t\t\t\t\t<ul>\n<li>Determinaci\u00f3n de la constante de afinidad (KD), cin\u00e9tica: constantes de asociaci\u00f3n y disociaci\u00f3n (kon, koff) en diferentes modelos (prote\u00edna-prote\u00edna, prote\u00edna-p\u00e9ptido, prote\u00edna-mol\u00e9cula peque\u00f1a, prote\u00edna-ARN &#8230;). Determinaci\u00f3n de la concentraci\u00f3n efectiva y control de calidad.\u00a0Isotipado de anticuerpos.\u00a0Cribado de posibles f\u00e1rmacos \/ biosimilares<\/li>\n<li>Cuantificaci\u00f3n de hasta 50 biomarcadores en muestras y fluidos biol\u00f3gicos en placa de 96 pocillos por Luminex<\/li>\n<li>Cuantificaci\u00f3n ultra-sensitiva de hasta 4 biomarcadores en muestras y fluidos biol\u00f3gicos en placa de 96 pocillos por SIMOA<\/li>\n<li>Medida de la tasa de consumo de ox\u00edgeno (OCR) y la tasa de acidificaci\u00f3n extracelular (ECAR) en placa de 96 pocillos (Seahorse): respiraci\u00f3n mitocondrial, gluc\u00f3lisis, producci\u00f3n de ATP, lip\u00f3lisis<\/li>\n<li>Transcript\u00f3mica y prote\u00f3mica espacial con resoluci\u00f3n de c\u00e9lula \u00fanica con CosMx (Nanostring). Ensayos de 6000 ARN, 1000 ARN o 64 prote\u00ednas en formato de 2 o 4 portaobjetos.<br \/>\nDise\u00f1o experimental, configuraci\u00f3n y optimizaci\u00f3n de las condiciones experimentales<\/li>\n<li>Apoyo cient\u00edfico-tecnol\u00f3gico a investigadores<\/li>\n<li>Capacitaci\u00f3n de nuevos usuarios en el uso del equipo.<\/li>\n<\/ul>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-1\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-1\">Aparatos<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-1\" tabindex=\"-1\"><i title=\"Expand\">Expand<\/i><\/a>\n\t\t\t\t\t<ul>\n<li>Biacore T-200 (GE) para an\u00e1lisis SPR de interacciones<\/li>\n<li>Analizador Seahorse XFe96 (Agilent) para la detecci\u00f3n de OCR y ECAR en una placa de 96 pocillos<\/li>\n<li>Magpix (Luminex) que incluye software xPonent 4.2 para an\u00e1lisis multiplex de hasta 50 perlas magn\u00e9ticas<\/li>\n<li>Sistema de detecci\u00f3n de biomarcadores ultra-sensitivo SIMOA SR-X (Quanterix)<\/li>\n<li>Plataforma de im\u00e1genes unicelulares multi\u00f3micas espaciales CosMx<\/li>\n<\/ul>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-2\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-2\">Publicaciones<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-2\" tabindex=\"-1\"><i title=\"Expand\">Expand<\/i><\/a>\n\t\t\t\t\t<p>The NADPH oxidase NOX4 regulates redox and metabolic homeostasis preventing HCC progression. Pe\u00f1uelas-Haro, I., Espinosa-Sotelo, R., Crosas-Molist, E., &#8230; Bertran, E. and Fabregat, I.\u00a0Hepatology 2023, 78, 416-433. (agra\u00efments Seahorse)<\/p>\n<p>A novel family of lysosomotropic tetracyclic compounds for treating leukemia. Carb\u00f3, J.M., Cornet-Masana, J.M., .. and Risue\u00f1o, R.M. Cancers 2023, 15, 1912 (agra\u00efments Seahorse)<\/p>\n<p>Dual and opposite costimulatory targeting with a novel human fusion recombinant protein effectively prevents renal warm ischemia reperfusion injury and allograft rejection in murine models. Guiteras, J, De Ramon, L., Crespo, E, Bola\u00f1os, N, <strong>Barcel\u00f3-Batllori, S<\/strong>., et al. International Journal of Molecular Sciences, 2021, 22, 1216<\/p>\n<p>Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis. Colom\u00e9, N., Abi\u00e1n, J., Aloria, K., Arizmendi, JM., <strong>Barcel\u00f3-Batllori, S<\/strong>., et al. Journal of Proteomics, 2022, 251, 104409<\/p>\n<p>Urinary cytokines reflect renal inflammation in acute tubulointerstitial nephritis: A multiplex bead-based assay assessment. Martinez Valenzulea, L., Draibe, J., Bestard, O., Fulladosa, X., et al. Journal of Clinical Medicine, 2021, 10, 2986 (agra\u00efments Luminex)<\/p>\n<p>Immunodominant proteins P1 and P40\/P90 from human pathogen Mycoplasma pneumoniae. Vizarraga, D., Kawamoto, A., Matsumoto J, Illanes, R., P\u00e9rez-Luque, R., et al. Nature Communications, 2020, 11, 5188 (agra\u00efments Biacore)<\/p>\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29317652\/\" target=\"_blank\" rel=\"noopener\">SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-kB pathway. <\/a><br \/>\nSantos-Barriopedro, I., Bosch-Presegu\u00e9, L., Marazuela-Duque, A., de la Torre, C., Colomer, C., Vazquez, B.N., Fuhrmann, T., Mart\u00ednez-Pastor, B., Lu, W., Braun, T., Bober, E., Jenuwein, T., Serrano, L., Esteller, M., Cheng, Z., <strong>Barcel\u00f3-Batllori, S<\/strong>., Mostoslavsky, R., Espinosa, Ll., &amp; Vaquero, A.<br \/>\nNature communications, 2018, 9:101. doi: 10.1038\/s41467-017-02586\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26208904\/\" target=\"_blank\" rel=\"noopener\">KAT6B is a tumor suppressor histone H3 lysine 23 acetyltransferase undergoing genomic loss in small cell lung cancer.<\/a><br \/>\nSim\u00f3-Riudalbas, L., P\u00e9rez-Salvia, M., Setien F., Villanueva, A., Moutinho, C., Mart\u00ednez-Card\u00fas, A., Moran, S., Berdasco, M., Gomez, A., Vidal, E., Soler, M., Heyn, H., Vaquero, A., de la Torre, C.,<strong> Barcel\u00f3-Batllori <\/strong>S, &#8230; et Esteller, M<br \/>\nCancer Research 2015, 75, 3936-3945\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/17986440\/\" target=\"_blank\" rel=\"noopener\">Integration of DIGE and bioinformatics analyses reveals a role of the anti-obesity agent tungstate in redox and energy homeostasis pathways in brown adipose tissue.<\/a><br \/>\n<strong>Barcel\u00f3-Batllori, S<\/strong>., Esteban, Y. Moreno, S., Kalko, S. and Gomis, R.<br \/>\nMolecular and Cellular Proteomics, 2008, 7, 378-393\n\t<p><div class=\"ast-oembed-container \" style=\"height: 100%;\"><iframe loading=\"lazy\" title=\"Els serveis cientificot\u00e8cnics de l&#039;IDIBELL\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/ZaXrvEbwE5U?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/div><\/p>\n<p>&nbsp;<\/p>\n<p>Descubre los servicios cientificot\u00e9cnicos del IDIBELL!<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/uploads\/sites\/4\/2022\/02\/silvia-150x150.jpg\" alt=\"silvia\" itemprop=\"image\" height=\"150\" width=\"150\" title=\"silvia\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\tSilvia Barcel\u00f3\n\t<\/h4>\n<h4>\n\t\tCONTACTA\n\t<\/h4>\n<h4>\n\t\t<a href=\"mailto:molecularinteractions@idibell.cat\" title=\"Interacciones Moleculares\"  target=\"_blank\"  rel=\"noopener\">\n\t\tInteracciones Moleculares\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\tDOCUMENTOS\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/drive.google.com\/file\/d\/1x-tU0KVraTtxeyS9Y6zVuT6b-VdxBJBc\/view?usp=sharing\" title=\"Tarifas Plataformas [pdf]\"  target=\"_blank\"  rel=\"noopener\">\n\t\tTarifas Plataformas [pdf]\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/es\/wp-content\/uploads\/sites\/4\/2020\/11\/SCT-MI-FOR-01001ENG-Request-Biacore-v1.docx\" title=\"Hoja Solicitud Biacore\"  target=\"_blank\"  rel=\"noopener\">\n\t\tHoja Solicitud Biacore\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/es\/wp-content\/uploads\/sites\/4\/2020\/11\/SCT-MI-FOR-01003ENG-Request-Luminex-v1.docx\" title=\"Hoja Solicitud Luminex\"  target=\"_blank\"  rel=\"noopener\">\n\t\tHoja Solicitud Luminex\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/es\/wp-content\/uploads\/sites\/4\/2020\/11\/SCT-MI-FOR-01004ENG-Request-Seahorse.docx\" title=\"Hoja Solicitud Seahorse\"  target=\"_blank\"  rel=\"noopener\">\n\t\tHoja Solicitud Seahorse\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/idibell.cat\/es\/wp-content\/uploads\/sites\/4\/2020\/11\/SCT-MI-FOR-01005ENG-Request-Typhoon.docx\" title=\"Hoja Solicitud Typhoon\"  target=\"_blank\"  rel=\"noopener\">\n\t\tHoja Solicitud Typhoon\n\t\t<\/a>\n\t<\/h4>\n<h2>\n\t\tEquipo\n\t<\/h2>\n\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-tabs--label-0\" data-index=\"0\" aria-selected=\"true\" aria-controls=\"fl-tabs--panel-0\" aria-expanded=\"true\" role=\"tab\" tabindex=\"0\">\t\t\t\tJefa de Plataforma\t\t\t\t<\/a>\n\t\t\t\t\t\tJefa de Plataforma\n\t\t\t\t\t\t\t\t\t\t\t<ul>\n<li>Silvia Barcel\u00f3 Batllori<\/li>\n<\/ul>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/es\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\n","protected":false},"excerpt":{"rendered":"<p>Interacciones Moleculares La Plataforma de Interacciones Moleculares proporciona apoyo cient\u00edfico-tecnol\u00f3gico a la comunidad cient\u00edfica en el campo de las interacciones moleculares y el descubrimiento de biomarcadores utilizando varios enfoques. En primer lugar, Surface Plasmon Resonance (SPR) permite la monitorizaci\u00f3n en tiempo real de cualquier tipo de interacciones moleculares (prote\u00edna-prote\u00edna, prote\u00edna-f\u00e1rmacos, prote\u00edna-ves\u00edculas, prote\u00edna-ARN,&#8230;) y realiza mediciones [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":679,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-764","page","type-page","status-publish","hentry"],"publishpress_future_action":{"enabled":false,"date":"2025-05-18 13:14:37","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/pages\/764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/comments?post=764"}],"version-history":[{"count":0,"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/pages\/764\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/pages\/679"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/es\/wp-json\/wp\/v2\/media?parent=764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}