Colonbiome<\/a> project. This wide project aims to find microbiota markers that can be used for the early detection of colon cancer. To do this, colon biopsies and fecal samples will be collected from healthy patients and patients in different stages of colorectal cancer. Then the microbiota\u2019s genome will be sequenced to identify differences between groups.<\/p>\n <\/p>\n
Data available to everyone<\/h4>\n
<\/p>\n
All the data obtained in this pilot study has been entered into the European Nucleotide Archive, a public and collaborative database, where all types of genomic sequences are shared for the benefit of the entire scientific community. In addition, all the results of this first pilot test have been published in the Scientific Data journal, also a public journal, where both the sequences and the bioinformatics analysis methods used are detailed.<\/p>\n
This study not only aims to be useful for the future work of the group, but it also aims to be helpful for all research groups that are carrying out similar analyses or trying new bioinformatics tools, who have open access to all the results obtained in this study. In addition, the researchers assure that they will also make public all the details of the subsequent studies, to continue contributing to collaboration and progress in the field.<\/p>\n
<\/p>\n
The two sequencing methods<\/h4>\n
<\/p>\n
Two sequencing methods were compared in the study: the 16s and the Shotgun. The first is focused on the sequence of a single gene of the microorganisms, while the second gives us the complete sequence of the entire genome. Although the sequencing of a single gene implicates less sensibility, it can be cheaper. Furthermore, sequencing a gene only present in the microbiota allows us to analyze biopsy samples without the interference of the human genome.<\/p>\n
Additionally, the pilot test has shown that both techniques are consistent. Although complete sequencing is more sensitive and can distinguish more species of microorganisms, the results are not contradictory to single-gene sequencing.<\/p>\n","protected":false},"excerpt":{"rendered":"
In this pilot study, they used colon biopsies samples and feces samples from nine patients to compare two sequencing methods. It has been defined the appropriate bioinformatic analysis methods for this type of study.<\/p>\n","protected":false},"author":6,"featured_media":7154,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[180,183],"tags":[],"class_list":["post-4892","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cancer","category-colorectal-cancer"],"publishpress_future_action":{"enabled":false,"date":"2024-09-14 18:40:02","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"_links":{"self":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/posts\/4892"}],"collection":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/comments?post=4892"}],"version-history":[{"count":0,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/posts\/4892\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/media\/7154"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/media?parent=4892"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/categories?post=4892"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/tags?post=4892"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}