{"id":952,"date":"2019-05-23T04:47:32","date_gmt":"2019-05-23T02:47:32","guid":{"rendered":"https:\/\/idibell.cat\/?page_id=952"},"modified":"2023-02-17T08:30:40","modified_gmt":"2023-02-17T07:30:40","slug":"mouse-and-embryo-micromanipulation","status":"publish","type":"page","link":"https:\/\/idibell.cat\/en\/services\/scientific-and-technical-services\/mouse-and-embryo-micromanipulation\/","title":{"rendered":"Mouse and embryo micromanipulation"},"content":{"rendered":"\n<h1>\n\t\tMouse Lab\n\t<\/h1>\nThe Mouse Lab is a platform designed to provide Idibell researchers the most advanced technology for the manipulation of the mouse embryo and offer expertise and scientific and technical support on several tools and techniques to allow Idibell researchers to optimize their research projects.<br \/>\nThe laboratory, located in the Idibell Animal Facility, counts on specialized personnel in continuous formation that allows maintaining the innovating capacity of the Idibell, as well as a specialized and personal attention to each research project.\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/uploads\/sites\/2\/2020\/03\/ratolins.jpg\" alt=\"ratolins\" itemprop=\"image\" height=\"300\" width=\"600\" title=\"ratolins\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-0\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-0\">Services<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-0\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-0\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<p>Techniques and projects with specialized personnel:<\/p>\n<ul>\n<li>Obtention and manipulation of mouse embryo 0.5dpc-15.5dpc.<\/li>\n<li>Transgenesis by injection of targeted ES cells into blastocysts.<\/li>\n<li>Embryo criopreservation.<\/li>\n<li>Embryo transfers and strain rederivation.<\/li>\n<li>Surgeries: Heart, kidney , vasectomies&#8230;<\/li>\n<li>Adult mice samples obtention.<\/li>\n<li>Embrionic tissues obtention.<\/li>\n<li>Blood samples obtention.<\/li>\n<li>ES cells injection for caracterization.<\/li>\n<li>ES cells injection for transplant i.f., i.r.o. or i.v tail injection.<\/li>\n<li>Hormonal stimulation of females.<\/li>\n<li>In vitro fertilization.<\/li>\n<li>Virus injection.<\/li>\n<li>Obtention of pregnant and pseudopregnant females.<\/li>\n<li>Teratoma injection, production and obtention. <\/li>\n<li>Gavage feeding. <\/li>\n<li>Animal welfare supervision.<\/li>\n<li>Management of different transgenic mice colonies.<\/li>\n<\/ul>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-1\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-1\">Equipment<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-1\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-1\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<ul>\n<li>IX71 Micromanipulation Station + TOKAI + Newport Table.<\/li>\n<li>OCTAX LASER SHOT (includes laser system + software + camera)<\/li>\n<li>Micromanipulation controllers with Cell Tram Vario<\/li>\n<li>Micromanipulation controllers with Cell Tram Air<\/li>\n<li>CO2 anesthesia trolley<\/li>\n<li>IX71 Microscope with anti-vibration table<\/li>\n<li>SZX12 magnifying glass with incident lighting (only from above)<\/li>\n<li>SZX12 loupe with fluorescence, heated stage and incident and transmitted illumination<\/li>\n<li>SZX12 magnifying glass with heated stage and incident and transmitted lighting<\/li>\n<li>Telstar BIO II-A biosafety cabinet<\/li>\n<li>CO2 incubators<\/li>\n<\/ul>\n\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--label-2\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-2\">Publications<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-accordion--icon-2\" tabindex=\"0\" aria-controls=\"fl-accordion--panel-2\"><i>Expand<\/i><\/a>\n\t\t\t\t\t<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29732402\/\" rel=\"noopener\" target=\"_blank\">The local microenvironment limits the regenerative potential of the mouse neonatal heart.<\/a><br \/>\nMario Notari, <strong>Antoni Ventura-Rubi<\/strong>o, Sylvia J. Bedford-Guaus, Ignasi Jorba, Lola Mulero, Daniel Navajas, Merc\u00e8 Mart\u00ed and \u00c1ngel Raya.<br \/>\nScience Advances 02 May 2018: Vol. 4, no. 5,  \n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27732845\/\" rel=\"noopener\" target=\"_blank\">Direct Conversion of Fibroblasts to Megakaryocyte Progenitors.<\/a><br \/>\nJulian Pulecioco, Oriol Alejo-Valle, Sandra Capellera-Garcia, Marianna Vitaloni, Paula Rio, Eva Mej\u00eda-Ram\u00edrez, Ilaria Caserta, Juan A. Bueren, Johan Flygare, Angel Raya.<br \/>\nCell Repprts. 2016 Oct 11;17(3):671-683 \n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27702419\/\" rel=\"noopener\" target=\"_blank\">Expression of the T85A mutant of zebrafish aquaporin 3b improves post-thaw survival of cryopreserve.<\/a><br \/>\nBedford-Guaus SJ, Chauvign\u00e9 F, Mej\u00eda-Ram\u00edrez E, Mart\u00ed M, <strong>Ventura-Rubio A, <\/strong>Raya A, Cerd\u00e0 J, Veiga A.<br \/>\nZygote. 2016 Dec;24(6):839-847. \n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/21981310\/\" rel=\"noopener\" target=\"_blank\">Increased dosage of tumor suppressors limits the tumorigenicity of iPS cells without affecting their pluripotency<\/a>.<br \/>\nSergio Menendez, Suzanne Camus, Aida Herreria, Ida Paramonov, Laura B. Morera, Manuel Collado, Vlad Pekarik, Iago Maceda , Michael Edel, Antonella Consiglio, Adriana Sanchez, Han Li, Manuel Serrano, Juan C. I. Belmonte.<br \/>\nAging Cell. 2012 Feb;11(1):41-50. \n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/24046443\/\" rel=\"noopener\" target=\"_blank\">M-cadherin-mediated intercellular interactions activate satellite cell division.<\/a><br \/>\nMerce Marti, N\u00faria Montserrat, Cristina Pardo, Lola Mulero, Laia Miquel-Serra, Alexandre Miguel Cavaco Rodrigues, Jos\u00e9 Andr\u00e9s Vaquero, Bernd Kuebler, Cristina Morera, Mar\u00eda Jos\u00e9 Barrero, Juan Carlos Izpisua Belmonte.<br \/>\nJ Cell Sci. 2013 Nov 15;126(Pt 22):5116-31 \n\t<p><div class=\"ast-oembed-container \" style=\"height: 100%;\"><iframe loading=\"lazy\" title=\"Scientific and technical services of IDIBELL\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/cLQtSMtOyCg?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/div><\/p>\n<p>&nbsp;<\/p>\n<p>Discover the IDIBELL scientific and technical services!<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/uploads\/sites\/2\/2022\/02\/juani-150x150.jpg\" alt=\"juani\" itemprop=\"image\" height=\"150\" width=\"150\" title=\"juani\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\tJuani Fernandez\n\t<\/h4>\n<h4>\n\t\tCONTACT\n\t<\/h4>\n<h4>\n\t\t<a href=\"mailto:mouse@idibell.cat\" title=\"Mouse Lab\" target=\"_blank\" rel=\"noopener\">\n\t\tMouse Lab\n\t\t<\/a>\n\t<\/h4>\n<h4>\n\t\tDOCUMENTS\n\t<\/h4>\n<h4>\n\t\t<a href=\"https:\/\/drive.google.com\/file\/d\/1v2V6HM77HkyaLqyQlLVJPJ56MmwUZ_cA\/view?usp=sharing\" title=\"Core Facilities Fees (pdf)\" target=\"_blank\" rel=\"noopener\">\n\t\tCore Facilities Fees (pdf)\n\t\t<\/a>\n\t<\/h4>\n<h2>\n\t\tTeam\n\t<\/h2>\n\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-tabs--label-0\" data-index=\"0\" aria-selected=\"true\" aria-controls=\"fl-tabs--panel-0\" aria-expanded=\"true\" role=\"tab\" tabindex=\"0\">\t\t\t\tHead of Platform\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t<a href=\"#\" id=\"fl-tabs--label-1\" data-index=\"1\" aria-selected=\"false\" aria-controls=\"fl-tabs--panel-1\" aria-expanded=\"false\" role=\"tab\" tabindex=\"0\">\t\t\t\tTeam\t\t\t\t<\/a>\n\t\t\t\t\t\tHead of Platform\n\t\t\t\t\t\t\t\t\t\t\t<ul>\n<li>Juani Fernandez<\/li>\n<\/ul>\n\t\t\t\t\t\tTeam\n\t\t\t\t\t\t\t\t\t\t\t<ul>\n<li>Antoni Ventura<\/li>\n<li>Alba Mart\u00ednez<\/li>\n<li>Marina Pe\u00f1afiel<\/li>\n<li>Aida Lopez<\/li>\n<\/ul>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/idibell.cat\/en\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\"  \/>\n\n","protected":false},"excerpt":{"rendered":"<p>Mouse Lab The Mouse Lab is a platform designed to provide Idibell researchers the most advanced technology for the manipulation of the mouse embryo and offer expertise and scientific and technical support on several tools and techniques to allow Idibell researchers to optimize their research projects. The laboratory, located in the Idibell Animal Facility, counts [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":679,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"publishpress_future_action":{"enabled":false,"date":"2024-05-13 13:25:49","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/pages\/952"}],"collection":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/comments?post=952"}],"version-history":[{"count":5,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/pages\/952\/revisions"}],"predecessor-version":[{"id":21947,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/pages\/952\/revisions\/21947"}],"up":[{"embeddable":true,"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/pages\/679"}],"wp:attachment":[{"href":"https:\/\/idibell.cat\/en\/wp-json\/wp\/v2\/media?parent=952"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}