Mouse Lab

Techniques and projects with specialized personnel:

• Obtention and manipulation of mouse embryo 0.5dpc-15.5dpc.
• Transgenesis by injection of targeted ES cells into blastocysts.
• Embryo criopreservation.
• Embryo transfers and strain rederivation.
• Surgeries: Heart, kidney , vasectomies…
• Adult mice samples obtention.
• Embrionic tissues obtention.
• Blood samples obtention.
• ES cells injection for caracterization.
• ES cells injection for transplant i.f., i.r.o. or i.v tail injection.
• Hormonal stimulation of females.
• In vitro fertilization.
• Virus injection.
• Obtention of pregnant and pseudopregnant females.
• Teratoma injection, production and obtention.
• Gavage feeding.
• Animal welfare supervision.
• Management of different transgenic mice colonies.

• IX71 Micromanipulation Station + TOKAI + Newport Table.
• OCTAX LASER SHOT (includes laser system + software + camera)
• Micromanipulation controllers with Cell Tram Vario
• Micromanipulation controllers with Cell Tram Air
• CO2 anesthesia trolley
• IX71 Microscope with anti-vibration table
• SZX12 magnifying glass with incident lighting (only from above)
• SZX12 loupe with fluorescence, heated stage and incident and transmitted illumination
• SZX12 magnifying glass with heated stage and incident and transmitted lighting
• Telstar BIO II-A biosafety cabinet
• CO2 incubators

The local microenvironment limits the regenerative potential of the mouse neonatal heart.
Mario Notari, Antoni Ventura-Rubio, Sylvia J. Bedford-Guaus, Ignasi Jorba, Lola Mulero, Daniel Navajas, Mercè Martí and Ángel Raya.
Science Advances 02 May 2018: Vol. 4, no. 5,

Direct Conversion of Fibroblasts to Megakaryocyte Progenitors.
Julian Pulecioco, Oriol Alejo-Valle, Sandra Capellera-Garcia, Marianna Vitaloni, Paula Rio, Eva Mejía-Ramírez, Ilaria Caserta, Juan A. Bueren, Johan Flygare, Angel Raya.
Cell Repprts. 2016 Oct 11;17(3):671-683

Expression of the T85A mutant of zebrafish aquaporin 3b improves post-thaw survival of cryopreserve.
Bedford-Guaus SJ, Chauvigné F, Mejía-Ramírez E, Martí M, Ventura-Rubio A, Raya A, Cerdà J, Veiga A.
Zygote. 2016 Dec;24(6):839-847.

Increased dosage of tumor suppressors limits the tumorigenicity of iPS cells without affecting their pluripotency.
Sergio Menendez, Suzanne Camus, Aida Herreria, Ida Paramonov, Laura B. Morera, Manuel Collado, Vlad Pekarik, Iago Maceda , Michael Edel, Antonella Consiglio, Adriana Sanchez, Han Li, Manuel Serrano, Juan C. I. Belmonte.
Aging Cell. 2012 Feb;11(1):41-50.

M-cadherin-mediated intercellular interactions activate satellite cell division.
Merce Marti, Núria Montserrat, Cristina Pardo, Lola Mulero, Laia Miquel-Serra, Alexandre Miguel Cavaco Rodrigues, José Andrés Vaquero, Bernd Kuebler, Cristina Morera, María José Barrero, Juan Carlos Izpisua Belmonte.
J Cell Sci. 2013 Nov 15;126(Pt 22):5116-31