Bioimaging

Optical Microscopy

• Design of experiments related to optical microscopy.
• Performance of advanced microscopy techniques (FRET, FRAP, live imaging, etc.).
• Training in the use of equipment.
• Image processing and analysis.

Electron Microscopy

• Support in the preparation of the sample, including the embedding and ultramicrotomy of Epon resin blocks both in cells and aggregates, as well as in tissues.
• Support in the acquisition and subsequent analysis of images.
• Proper training so that users can use the microscopes on their own during the working day.

Optical Microscopy

• 2 Inverted confocal microscopes Leica TCS SP5 AOBS equipped with motorized stage and incubation chamber.
• Leica TCS SPE confocal microscope.
• Zeiss Observer Z1 inverted widefield microscope equipped with motorized stage, incubation chamber and Apotome structured illumination system.
• Olympus IX81 Cell R inverted widefield microscope equipped with motorized stage and incubation chamber.
• 2 Leica DM6000 widefield microscopes.
• Olympus IX81 inverted widefield microscope.
• Nikon Eclipse 80i widefield microscope.
• Olympus BX51 widefield microscope.
• Leica MZ16 stereo microscope equipped with fluorescence module.
• Leica Z16APO stereo microscope equipped with fluorescence module.
• Olympus MVX10 stereo microscope.
• Image analysis software: Imaris, Fiji (ImageJ), LAS AF, LASX.

Electron Microscopy

• 1 Transmission Electron Microscope/TEM – Jeol JEM-1011
• 1 Scanning Electron Microscope/SEM – Jeol JSM-6390LV
• 1 Cryotissue processor/robot – Leica AFS2
• 1 Ultramicrotome – Leica EMUC6
• 1 Glass knife Machine – Leica EMKMR2
• 1 Critical point – EMTECH K850
• 1 Gold metallizer – EMTECH K550X
• 1 Hotplate – Stuart CB300
• 1 Optical microscope – Leica DM1000
• 2 Steromicroscope - Leica S6E + Light source Leica L2
• 1 Fume hood – Burdinola B ST 1500 LOW

Characterization of pluripotent stem cells.
Mercè Martí, Lola Mulero, Cristina Pardo, Cristina Morera, Meritxell Carrió, Leopoldo Laricchia-Robbio, Concepcion Rodriguez Esteban & Juan Carlos Izpisúa Belmonte. Nature Protocols vol. 8, pages 223–253. 10 January 2013; doi:10.1038/nprot.2012.154

Mcad-mediated intercellular interactions activate satellite cell división.
Merce Marti, Núria Montserrat, Cristina Pardo, Lola Mulero, Laia Miquel-Serra, Alexandre Miguel Cavaco Rodrigues, José Andrés Vaquero, Bernd Kuebler, Cristina Morera, María José Barrero & Juan Carlos Izpisúa Belmonte. J Cell Sci. 17 September 2013; doi: 10.1242/jcs.123562