Advanced Cell and Tissue Cultures

• Pluripotent Stem Cell Culture Training
• IPSC generation by different methodologies
• Support in “in vitro” differentiation in 2D and 3D to different cell types
• Support in obtaining organoids
• Genomic editing using CRISPR / Cas9
• Development of new related protocols
• Generation of Irradiated Mouse embryonic fibroblasts (iMEFS) cryovials
• Generation of Irradiated Human Foreskin fibroblasts (iHFF) cryovials
• Mycoplasma test
• SARS-CoV-2 detection in untested research human sample

IDIBELL premises include a spacious, state-of-the-art, self-contained cell and tissue culture unit which houses all equipment needed for growing, maintaining and analyzing cells. The Unit comprises 3 culture rooms of containment level II biosecurity (Clean cell culture room, Quarantine-Primary cell culture room and Decontamination cell culture room). In total the platform has 7 laminar flow hoods for the sterile handling of cells, as well as 12 atmosphere-controlled incubators for growing cells. Several inverted phase-contrast and standard bright-field microscopes and a fluorescence inverted microscope are available for cell analysis