Advanced Cell and Tissue Cultures

The Advanced Cell and Tissue Culture platform provides users with all the necessary equipment to perform cell cultures. It is divided into three culture rooms: a clean culture room, primary culture room, and decontamination or quarantine room. These three rooms are available to users in self-service mode or with technical support.

The platform staff offers their technical support to researchers both in standardized cell culture protocols (maintenance of cell lines, derivation of primary tissue, cell cryopreservation, etc.) and in the development of the requested cell culture experiment. Also, it offers its support in the experimental design, manipulation or processing of the sample, and the analysis of results.

The platform also participates in training activities, giving courses on cell cultures of different levels of complexity.

Following good laboratory practices, room cleaning routines, equipment maintenance, and quality controls are applied to guarantee an aseptic area and the proper functioning of the facilities.

Services

Maintenance of cell lines in culture
Derivation and establishment of primary cultures
Cell cryopreservation
Development and tuning of tests using cell cultures at the request of researchers
Genetic Editing using Crispr/Cas9
Mycoplasma test
Detection of SARS-CoV-2 in human samples
Generation of cryovials from irradiated Mouse Embryonic Fibroblasts (iMEFS)
Generation of Cryovials from irradiated Human Foreskin Fibroblasts (iHFF)
Training courses in cell cultures

Equipment

IDIBELL premises include a spacious, state-of-the-art, self-contained cell and tissue culture unit which houses all equipment needed for growing, maintaining and analyzing cells. The Unit comprises 3 culture rooms of containment level II biosecurity (Clean cell culture room, Quarantine-Primary cell culture room and Decontamination cell culture room). In total the platform has 7 laminar flow hoods for the sterile handling of cells, as well as 12 atmosphere-controlled incubators for growing cells. Several inverted phase-contrast and standard bright-field microscopes and a fluorescence inverted microscope are available for cell analysis